Exercise improves lipid droplet metabolism disorder through activation of AMPK-mediated lipophagy in NAFLD.
Life Sci
; 273: 119314, 2021 May 15.
Article
em En
| MEDLINE
| ID: mdl-33667513
ABSTRACT
AIM:
To emphasize the mechanism of the effect of exercise on lipid droplet (LD) metabolism disorder in nonalcoholic fatty liver disease (NAFLD). MAINMETHODS:
C57BL/6J mice were randomly divided into three groups The first group was fed with a normal diet (CON), the second group was fed a high-fat diet (HF), and finally group with a high-fat diet intervention and swim training (HF-EX). The total intervention period was 16 weeks. RT-PCR and Western blot were performed to evaluate the effect of exercise on LDs metabolism and the AMPK pathway. Histopathological examinations and immunofluorescence were performed to evaluate the lipid deposition and lipophagy in the liver. KEYFINDINGS:
Exercise reduced liver steatosis and insulin resistance along with the stimulation of AMPK/SIRT1 signaling and downstream regulation of lipid metabolism. In addition, exercise increased the expression of autophagy marker and colocalization of LC3 and LAMP1 with LDs.SIGNIFICANCE:
Exercise stimulated AMPK/SIRT1 and activated lipophagy in NAFLD. Enhancing lipophagy may be one of the key mechanisms of regulation and resolution of NAFLD by exercise.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Condicionamento Físico Animal
/
Autofagia
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Metabolismo dos Lipídeos
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Proteínas Quinases Ativadas por AMP
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Dieta Hiperlipídica
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Gotículas Lipídicas
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Hepatopatia Gordurosa não Alcoólica
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
En
Revista:
Life Sci
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
China