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Complete Freund's adjuvant-free experimental autoimmune encephalomyelitis in Dark Agouti rats is a valuable tool for multiple sclerosis studies.
Lazarevic, Milica; Djedovic, Neda; Stanisavljevic, Suzana; Dimitrijevic, Mirjana; Stegnjaic, Goran; Krishnamoorthy, Gurumoorthy; Mostarica Stojkovic, Marija; Miljkovic, Dorde; Jevtic, Bojan.
Afiliação
  • Lazarevic M; Department of Immunology, Institute for Biological Research "Sinisa Stankovic" - National Institute of Republic of Serbia, University of Belgrade, Despota Stefana 142, 11000 Belgrade, Serbia.
  • Djedovic N; Department of Immunology, Institute for Biological Research "Sinisa Stankovic" - National Institute of Republic of Serbia, University of Belgrade, Despota Stefana 142, 11000 Belgrade, Serbia.
  • Stanisavljevic S; Department of Immunology, Institute for Biological Research "Sinisa Stankovic" - National Institute of Republic of Serbia, University of Belgrade, Despota Stefana 142, 11000 Belgrade, Serbia.
  • Dimitrijevic M; Department of Immunology, Institute for Biological Research "Sinisa Stankovic" - National Institute of Republic of Serbia, University of Belgrade, Despota Stefana 142, 11000 Belgrade, Serbia.
  • Stegnjaic G; Department of Immunology, Institute for Biological Research "Sinisa Stankovic" - National Institute of Republic of Serbia, University of Belgrade, Despota Stefana 142, 11000 Belgrade, Serbia.
  • Krishnamoorthy G; Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.
  • Mostarica Stojkovic M; Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Dr Subotica 1, 11000 Belgrade, Serbia.
  • Miljkovic D; Department of Immunology, Institute for Biological Research "Sinisa Stankovic" - National Institute of Republic of Serbia, University of Belgrade, Despota Stefana 142, 11000 Belgrade, Serbia. Electronic address: djordjem@ibiss.bg.ac.rs.
  • Jevtic B; Department of Immunology, Institute for Biological Research "Sinisa Stankovic" - National Institute of Republic of Serbia, University of Belgrade, Despota Stefana 142, 11000 Belgrade, Serbia.
J Neuroimmunol ; 354: 577547, 2021 05 15.
Article em En | MEDLINE | ID: mdl-33765502
ABSTRACT
Experimental autoimmune encephalomyelitis (EAE) is classically induced with complete Freund's adjuvant (CFA). The immune response against CFA has a confounding influence on the translational capacity of EAE as a multiple sclerosis model. Here, we compare clinical, cellular and molecular properties between syngeneic spinal cord homogenate (SCH)- and SCH + CFA-immunized Dark Agouti rats. EAE signs were observed earlier and the cumulative clinical score was higher without CFA. Also, a higher number of immune cells infiltrates in the spinal cords was noticed at the peak of EAE without CFA. High spinal cord abundance of CD8+CD11bc+MHC class II+ cells was detected in SCH-immunized rats. Myelin basic protein -specific response can be elicited in the cells from the lymph nodes draining the site of SCH immunization. This CFA-free EAE is a reliable multiple sclerosis model.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Imunização / Encefalomielite Autoimune Experimental Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: J Neuroimmunol Ano de publicação: 2021 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Imunização / Encefalomielite Autoimune Experimental Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: J Neuroimmunol Ano de publicação: 2021 Tipo de documento: Article