Robustness of a Cancer Profiling Test Using Formalin-fixed Paraffin Embedded Tumor Specimens.
Anticancer Res
; 41(3): 1341-1348, 2021 Mar.
Article
em En
| MEDLINE
| ID: mdl-33788725
ABSTRACT
BACKGROUND/AIM:
Cancer profiling tests using formalin-fixed paraffin-embedded (FFPE) specimens with various conditions have become an essential tool for cancer treatment. The robustness of these tests needs to be addressed. MATERIALS ANDMETHODS:
A cancer profiling test, NCC oncopanel, was tested with FFPE specimens from various tissues with different storage conditions and fixation lengths. Next generation sequencing was performed with Miseq and the data were assembled using the human reference genome hg19.RESULTS:
Duration of storage and fixation affected the mapping statistics. Prolonged storage increased outward read paring and longer fixation rates caused increased singletons and unmapped reads.CONCLUSION:
Our results indicate that a cancer profiling test with target capturing method, NCC oncopanel, shows robustness for FFPE cancer specimens with various storage conditions.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Manejo de Espécimes
/
Fixação de Tecidos
/
Inclusão em Parafina
/
Perfilação da Expressão Gênica
/
Sequenciamento de Nucleotídeos em Larga Escala
/
Neoplasias
Limite:
Humans
Idioma:
En
Revista:
Anticancer Res
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Japão