Flavonoids-rich extract from Bidens bipinnata L. protects pancreatic ß-cells against oxidative stress-induced apoptosis through intrinsic and extrinsic pathways.
J Ethnopharmacol
; 275: 114097, 2021 Jul 15.
Article
em En
| MEDLINE
| ID: mdl-33823164
ABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE As a traditional Chinese medicinal, Bidens bipinnata L. has been used to treat many diseases with a long history in China. The anti-diabetic effects of extract from B. bipinnata have been demonstrated in the previous reports. AIM OF THE STUDY The protective effects of flavonoids-rich extract from B. bipinnata (BBTF) on cell damage induced by H2O2 in pancreatic ß cell and its potential mechanisms were evaluated. MATERIALS AND METHODS:
MTT, ROS production, nuclear staining and flow cytometry assays were adopted to determine the effects of BBTF on cell viability, production of ROS and cell apoptosis in H2O2-treated INS-1 cell. Cell apoptosis-related proteins expressions were detected by Western blot assay.RESULTS:
Pre-treatment of BBTF could significantly increase INS-1 cell viability, inhibit the production of intracellular ROS and reduced the characteristic features of cell apoptosis induced by H2O2 in INS-1 cells. The studies of the underlying mechanism showed that BBTF could regulate Bax and Bcl-2 proteins expressions, suppress the phosphorylation of JNK, ERK and p38, as well as down-regulate Fas and FasL proteins expressions induced by H2O2. The expressions of caspase-8, caspase-9 and caspase-3 were therefore decreased.CONCLUSION:
The results indicated that flavonoids-rich extract from B. bipinnata could be a natural agent in diabetic prevention and therapy.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Flavonoides
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Medicamentos de Ervas Chinesas
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Apoptose
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Estresse Oxidativo
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Bidens
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Células Secretoras de Insulina
Limite:
Animals
Idioma:
En
Revista:
J Ethnopharmacol
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
China