Immunological similarities between microsomal, cytosolic and nuclear progesterone receptors in the chick oviduct.
J Steroid Biochem
; 30(1-6): 329-32, 1988.
Article
em En
| MEDLINE
| ID: mdl-3386261
ABSTRACT
Progesterone receptor of microsomal, cytosolic and nuclear fractions of the chick oviduct was studied by using biochemical, immunochemical and immunohistochemical analyses. In the oviducts of estrogen-treated immature chicks cytosolic, microsomal and nuclear PR were 90, 9.6 and 0.4% of the total binding, respectively, whereas the corresponding values 1 h after progesterone administration were 33, 6 and 61%, respectively. Progesterone decreased the cytosolic and microsomal PR 90 and 88%, respectively. All the receptor forms were similarly recognized by anti-PR-IgG raised against B-subunit of the PR. By using a sensitive immunoelectron microscopy in most cells of the oviduct only nuclear PR antigen was detected both in estrogen-treated and estrogen-progesterone-treated chick oviductal cells. In most cells no PR was found in the cytoplasm nor in the microsomes. Occasionally in very few cells small amounts of PR were found, associated with rough endoplasmic reticulum close to the nucleus containing a high concentration of the PR. This is probably due to a nascent synthesis of the PR. It is concluded that the major part of the cytosolic as well as microsomal PR is due to a homogenization artefact caused by a redistribution of the unoccupied PR located in the nuclei in situ.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oviductos
/
Receptores de Progesterona
/
Núcleo Celular
/
Microssomos
/
Complexo Antígeno-Anticorpo
Limite:
Animals
Idioma:
En
Revista:
J Steroid Biochem
Ano de publicação:
1988
Tipo de documento:
Article
País de afiliação:
Finlândia