Your browser doesn't support javascript.
loading
Diverse Dyes-Embedded Staphylococcus aureus as Potential Biocarriers for Enhancing Sensitivity in Biosensing.
Bu, Tong; Zhao, Shuang; Bai, Feier; Sun, Xinyu; He, Kunyi; Wang, Qinzhi; Jia, Pei; Tian, Yongming; Zhang, Meng; Wang, Li.
Afiliação
  • Bu T; College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China.
  • Zhao S; College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China.
  • Bai F; College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China.
  • Sun X; College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China.
  • He K; College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China.
  • Wang Q; College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China.
  • Jia P; College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China.
  • Tian Y; College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China.
  • Zhang M; College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China.
  • Wang L; College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China.
Anal Chem ; 93(17): 6731-6738, 2021 05 04.
Article em En | MEDLINE | ID: mdl-33877823
Nanomaterials-based immunochromatographic assays (ICAs) have gained great commercial success in real-life point-of-care testing (POCT). Exploring novel carriers of ICAs with improved signaling and sustained activity favors the development of sensitive POCT. Herein a potent signal biotag, colored Staphylococcus aureus (SA), was created for ICA carriers through a mild self-assembly strategy, providing high luminance and abundant specific binding sites for immobilization of monoclonal antibodies (mAbs). The biocompatible SA-dyes (SADs) retained both an intact surface structure for mAbs labeling (Fc portion) and the superior bioactivity of immobilized mAbs (affinity constant was about 109 M-1), thus waiving the intrinsic limitations of traditional nanomaterials and endowing high sensitivity. Proof-of-concept was demonstrated by employing Congo red- or/and fluorescein isothiocyanate-embedded SA (SACR, SAFITC, and SACR-SAFITC) as ICA carriers to detect zearalenone (ZEN) through colorimetric or/and fluorimetric signals. Furthermore, the ICAs satisfied the clinical requirement perfectly, including limit of detection (0.013 ng/mL, which was at least an 85-fold improvement over that of traditional gold nanoparticles-based ICA), linearity (R2 > 0.98), reproducibility (RSD < 8%), selectivity, and stability. Importantly, the proposed biosensors could be well-applied in four real samples for ZEN monitoring with satisfactory recoveries, correlating well with the results from liquid chromatography-tandem mass spectrometry (LC-MS). This work also proved a universal design for tailoring coloration bands for SAD-ICA detection of multiple analytes.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Zearalenona / Nanopartículas Metálicas Tipo de estudo: Diagnostic_studies Idioma: En Revista: Anal Chem Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Zearalenona / Nanopartículas Metálicas Tipo de estudo: Diagnostic_studies Idioma: En Revista: Anal Chem Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China