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Lepidopteran wing scales contain abundant cross-linked film-forming histidine-rich cuticular proteins.
Liu, Jianqiu; Chen, Zhiwei; Xiao, Yingdan; Asano, Tsunaki; Li, Shenglong; Peng, Li; Chen, Enxiang; Zhang, Jiwei; Li, Wanshun; Zhang, Yan; Tong, Xiaoling; Kadono-Okuda, Keiko; Zhao, Ping; He, Ningjia; Arunkumar, Kallare P; Gopinathan, Karumathil P; Xia, Qingyou; Willis, Judith H; Goldsmith, Marian R; Mita, Kazuei.
Afiliação
  • Liu J; State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
  • Chen Z; Biological Science Research Center, Southwest University, Chongqing, China.
  • Xiao Y; State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
  • Asano T; State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
  • Li S; Biological Science Research Center, Southwest University, Chongqing, China.
  • Peng L; Department of Biological Sciences, Tokyo Metropolitan University, Tokyo, Japan.
  • Chen E; State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
  • Zhang J; Shanghai Center for Plant Stress Biology and Center of Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China.
  • Li W; State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
  • Zhang Y; Biological Science Research Center, Southwest University, Chongqing, China.
  • Tong X; State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
  • Kadono-Okuda K; State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
  • Zhao P; Biological Science Research Center, Southwest University, Chongqing, China.
  • He N; State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
  • Arunkumar KP; Biological Science Research Center, Southwest University, Chongqing, China.
  • Gopinathan KP; Biological Science Research Center, Southwest University, Chongqing, China.
  • Xia Q; Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan.
  • Willis JH; Biological Science Research Center, Southwest University, Chongqing, China.
  • Goldsmith MR; State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
  • Mita K; State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
Commun Biol ; 4(1): 491, 2021 04 22.
Article em En | MEDLINE | ID: mdl-33888855
Scales are symbolic characteristic of Lepidoptera; however, nothing is known about the contribution of cuticular proteins (CPs) to the complex patterning of lepidopteran scales. This is because scales are resistant to solubilization, thus hindering molecular studies. Here we succeeded in dissolving developing wing scales from Bombyx mori, allowing analysis of their protein composition. We identified a distinctive class of histidine rich (His-rich) CPs (6%-45%) from developing lepidopteran scales by LC-MS/MS. Functional studies using RNAi revealed CPs with different histidine content play distinct and critical roles in constructing the microstructure of the scale surface. Moreover, we successfully synthesized films in vitro by crosslinking a 45% His-rich CP (BmorCPR152) with laccase2 using N-acetyl- dopamine or N-ß-alanyl-dopamine as the substrate. This molecular study of scales provides fundamental information about how such a fine microstructure is constructed and insights into the potential application of CPs as new biomaterials.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bombyx / Asas de Animais / Proteínas / Proteínas de Insetos / Escamas de Animais Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Commun Biol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bombyx / Asas de Animais / Proteínas / Proteínas de Insetos / Escamas de Animais Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Commun Biol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China