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Transmission, infectivity, and neutralization of a spike L452R SARS-CoV-2 variant.
Deng, Xianding; Garcia-Knight, Miguel A; Khalid, Mir M; Servellita, Venice; Wang, Candace; Morris, Mary Kate; Sotomayor-González, Alicia; Glasner, Dustin R; Reyes, Kevin R; Gliwa, Amelia S; Reddy, Nikitha P; Sanchez San Martin, Claudia; Federman, Scot; Cheng, Jing; Balcerek, Joanna; Taylor, Jordan; Streithorst, Jessica A; Miller, Steve; Sreekumar, Bharath; Chen, Pei-Yi; Schulze-Gahmen, Ursula; Taha, Taha Y; Hayashi, Jennifer M; Simoneau, Camille R; Kumar, G Renuka; McMahon, Sarah; Lidsky, Peter V; Xiao, Yinghong; Hemarajata, Peera; Green, Nicole M; Espinosa, Alex; Kath, Chantha; Haw, Monica; Bell, John; Hacker, Jill K; Hanson, Carl; Wadford, Debra A; Anaya, Carlos; Ferguson, Donna; Frankino, Phillip A; Shivram, Haridha; Lareau, Liana F; Wyman, Stacia K; Ott, Melanie; Andino, Raul; Chiu, Charles Y.
Afiliação
  • Deng X; Department of Laboratory Medicine, University of California, San Francisco, San Francisco, CA 94158, USA; UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, CA 94158, USA.
  • Garcia-Knight MA; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA.
  • Khalid MM; Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA; Gladstone Institute of Virology, San Francisco, CA 94158, USA.
  • Servellita V; Department of Laboratory Medicine, University of California, San Francisco, San Francisco, CA 94158, USA; UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, CA 94158, USA.
  • Wang C; Department of Laboratory Medicine, University of California, San Francisco, San Francisco, CA 94158, USA; UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, CA 94158, USA.
  • Morris MK; California Department of Public Health, Richmond, CA 94804, USA.
  • Sotomayor-González A; Department of Laboratory Medicine, University of California, San Francisco, San Francisco, CA 94158, USA; UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, CA 94158, USA.
  • Glasner DR; Department of Laboratory Medicine, University of California, San Francisco, San Francisco, CA 94158, USA; UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, CA 94158, USA.
  • Reyes KR; Department of Laboratory Medicine, University of California, San Francisco, San Francisco, CA 94158, USA; UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, CA 94158, USA.
  • Gliwa AS; Department of Laboratory Medicine, University of California, San Francisco, San Francisco, CA 94158, USA; UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, CA 94158, USA.
  • Reddy NP; Department of Laboratory Medicine, University of California, San Francisco, San Francisco, CA 94158, USA; UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, CA 94158, USA.
  • Sanchez San Martin C; Department of Laboratory Medicine, University of California, San Francisco, San Francisco, CA 94158, USA; UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, CA 94158, USA.
  • Federman S; Laboratory for Genomics Research, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Cheng J; Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.
  • Balcerek J; Department of Laboratory Medicine, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Taylor J; Department of Laboratory Medicine, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Streithorst JA; Department of Laboratory Medicine, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Miller S; Department of Laboratory Medicine, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Sreekumar B; Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA; Gladstone Institute of Virology, San Francisco, CA 94158, USA.
  • Chen PY; Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA; Gladstone Institute of Virology, San Francisco, CA 94158, USA.
  • Schulze-Gahmen U; Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA; Gladstone Institute of Virology, San Francisco, CA 94158, USA.
  • Taha TY; Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA; Gladstone Institute of Virology, San Francisco, CA 94158, USA.
  • Hayashi JM; Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA; Gladstone Institute of Virology, San Francisco, CA 94158, USA.
  • Simoneau CR; Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA; Gladstone Institute of Virology, San Francisco, CA 94158, USA.
  • Kumar GR; Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA; Gladstone Institute of Virology, San Francisco, CA 94158, USA.
  • McMahon S; Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA; Gladstone Institute of Virology, San Francisco, CA 94158, USA.
  • Lidsky PV; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA.
  • Xiao Y; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA.
  • Hemarajata P; Los Angeles County Public Health Laboratories, Downey, CA 90242, USA.
  • Green NM; Los Angeles County Public Health Laboratories, Downey, CA 90242, USA.
  • Espinosa A; California Department of Public Health, Richmond, CA 94804, USA.
  • Kath C; California Department of Public Health, Richmond, CA 94804, USA.
  • Haw M; California Department of Public Health, Richmond, CA 94804, USA.
  • Bell J; California Department of Public Health, Richmond, CA 94804, USA.
  • Hacker JK; California Department of Public Health, Richmond, CA 94804, USA.
  • Hanson C; California Department of Public Health, Richmond, CA 94804, USA.
  • Wadford DA; California Department of Public Health, Richmond, CA 94804, USA.
  • Anaya C; Monterey County Department of Public Health, Monterey, CA 93906, USA.
  • Ferguson D; Monterey County Department of Public Health, Monterey, CA 93906, USA.
  • Frankino PA; Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Shivram H; Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Lareau LF; Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Wyman SK; Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Ott M; Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA; Gladstone Institute of Virology, San Francisco, CA 94158, USA; Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA. Electronic address: melanie.ott@gladstone.ucsf.edu
  • Andino R; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA. Electronic address: raul.andino@ucsf.edu.
  • Chiu CY; Department of Laboratory Medicine, University of California, San Francisco, San Francisco, CA 94158, USA; UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, CA 94158, USA; Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA; Innovative Genomi
Cell ; 184(13): 3426-3437.e8, 2021 06 24.
Article em En | MEDLINE | ID: mdl-33991487
ABSTRACT
We identified an emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variant by viral whole-genome sequencing of 2,172 nasal/nasopharyngeal swab samples from 44 counties in California, a state in the western United States. Named B.1.427/B.1.429 to denote its two lineages, the variant emerged in May 2020 and increased from 0% to >50% of sequenced cases from September 2020 to January 2021, showing 18.6%-24% increased transmissibility relative to wild-type circulating strains. The variant carries three mutations in the spike protein, including an L452R substitution. We found 2-fold increased B.1.427/B.1.429 viral shedding in vivo and increased L452R pseudovirus infection of cell cultures and lung organoids, albeit decreased relative to pseudoviruses carrying the N501Y mutation common to variants B.1.1.7, B.1.351, and P.1. Antibody neutralization assays revealed 4.0- to 6.7-fold and 2.0-fold decreases in neutralizing titers from convalescent patients and vaccine recipients, respectively. The increased prevalence of a more transmissible variant in California exhibiting decreased antibody neutralization warrants further investigation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Anticorpos Neutralizantes / Glicoproteína da Espícula de Coronavírus / SARS-CoV-2 / COVID-19 Tipo de estudo: Risk_factors_studies Limite: Humans Idioma: En Revista: Cell Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Anticorpos Neutralizantes / Glicoproteína da Espícula de Coronavírus / SARS-CoV-2 / COVID-19 Tipo de estudo: Risk_factors_studies Limite: Humans Idioma: En Revista: Cell Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos