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L1CAM is not associated with extracellular vesicles in human cerebrospinal fluid or plasma.
Norman, Maia; Ter-Ovanesyan, Dmitry; Trieu, Wendy; Lazarovits, Roey; Kowal, Emma J K; Lee, Ju Hyun; Chen-Plotkin, Alice S; Regev, Aviv; Church, George M; Walt, David R.
Afiliação
  • Norman M; Department of Pathology, Brigham and Women's Hospital, Boston, MA, USA.
  • Ter-Ovanesyan D; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Trieu W; Tufts University School of Medicine, Boston, MA, USA.
  • Lazarovits R; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Kowal EJK; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Lee JH; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Chen-Plotkin AS; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Regev A; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Church GM; Department of Neurology, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USA.
  • Walt DR; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
Nat Methods ; 18(6): 631-634, 2021 06.
Article em En | MEDLINE | ID: mdl-34092791
ABSTRACT
L1CAM is a transmembrane protein expressed on neurons that was presumed to be found on neuron-derived extracellular vesicles (NDEVs) in human biofluids. We developed a panel of single-molecule array assays to evaluate the use of L1CAM for NDEV isolation. We demonstrate that L1CAM is not associated with extracellular vesicles in human plasma or cerebrospinal fluid and therefore recommend against its use as a marker in NDEV isolation protocols.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Molécula L1 de Adesão de Célula Nervosa / Vesículas Extracelulares Tipo de estudo: Risk_factors_studies Limite: Humans Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
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Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Molécula L1 de Adesão de Célula Nervosa / Vesículas Extracelulares Tipo de estudo: Risk_factors_studies Limite: Humans Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos