ß3-Adrenergic receptors regulate human brown/beige adipocyte lipolysis and thermogenesis.
JCI Insight
; 6(11)2021 06 08.
Article
em En
| MEDLINE
| ID: mdl-34100382
ABSTRACT
ß3-Adrenergic receptors (ß3-ARs) are the predominant regulators of rodent brown adipose tissue (BAT) thermogenesis. However, in humans, the physiological relevance of BAT and ß3-AR remains controversial. Herein, using primary human adipocytes from supraclavicular neck fat and immortalized brown/beige adipocytes from deep neck fat from 2 subjects, we demonstrate that the ß3-AR plays a critical role in regulating lipolysis, glycolysis, and thermogenesis. Silencing of the ß3-AR compromised genes essential for thermogenesis, fatty acid metabolism, and mitochondrial mass. Functionally, reduction of ß3-AR lowered agonist-mediated increases in intracellular cAMP, lipolysis, and lipolysis-activated, uncoupling protein 1-mediated thermogenic capacity. Furthermore, mirabegron, a selective human ß3-AR agonist, stimulated BAT lipolysis and thermogenesis, and both processes were lost after silencing ß3-AR expression. This study highlights that ß3-ARs in human brown/beige adipocytes are required to maintain multiple components of the lipolytic and thermogenic cellular machinery and that ß3-AR agonists could be used to achieve metabolic benefit in humans.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptores Adrenérgicos beta 3
/
Termogênese
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Adipócitos Marrons
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Adipócitos Bege
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Lipólise
Limite:
Humans
Idioma:
En
Revista:
JCI Insight
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Estados Unidos