Induction of an antibody that catalyzes the hydrolysis of an amide bond.
Science
; 241(4870): 1188-91, 1988 Sep 02.
Article
em En
| MEDLINE
| ID: mdl-3413482
ABSTRACT
Catalysis of amide bond hydrolysis is of singular importance in enzymology. An antibody was induced to an analog of a high-energy intermediate anticipated along the reaction coordinate of amide hydrolysis. This antibody is an amidase with high specificity and a large rate enhancement (250,000) relative to the uncatalyzed reaction. This reaction represents the kinetically most difficult hydrolysis reaction yet catalyzed by an antibody.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Catálise
/
Anticorpos Monoclonais
Limite:
Animals
Idioma:
En
Revista:
Science
Ano de publicação:
1988
Tipo de documento:
Article