Activation of 8-17 DNAzyme with extra functional group at conserved residues is related to catalytic metal ion.

Zhang, Wenjie; Li, Yang; Du, Shanshan; Chai, Zhilong; He, Junlin

Zhang, Wenjie; Li, Yang; Du, Shanshan; Chai, Zhilong; He, Junlin.

Afiliação

Zhang W; School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Li Y; State Key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology & Toxicology, Beijing 100850, China.
Du S; State Key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology & Toxicology, Beijing 100850, China.
Chai Z; State Key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology & Toxicology, Beijing 100850, China.
He J; State Key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology & Toxicology, Beijing 100850, China. Electronic address: hejunlin@bmi.ac.cn.

Bioorg Med Chem Lett ; 48: 128234, 2021 09 15.

Article em En | MEDLINE | ID: mdl-34214510

ABSTRACT

ABSTRACT

In 8-17 DNAzyme, the end loop A6G7C8 is a highly conserved motif. Here we reported an activation approach by specific chemical modifications on A6 and C8 for more efficient Ca2+-mediated reaction. The importance of the end loop was further highlighted and its critical conservation broken for more powerful catalysts.

Assuntos

Cálcio/metabolismo; DNA Catalítico/metabolismo; Cálcio/química; Catálise; DNA Catalítico/química; Estrutura Molecular

Palavras-chave

817 DNAzyme; Catalytic core; Functional group; Metal ion dependence; Observed rate constant

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cálcio / DNA Catalítico Idioma: En Revista: Bioorg Med Chem Lett Assunto da revista: BIOQUIMICA / QUIMICA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China

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