Cytogenetic and molecular study of two human neuroblastoma cell lines.
Cancer Genet Cytogenet
; 30(2): 225-31, 1988 Feb.
Article
em En
| MEDLINE
| ID: mdl-3422578
ABSTRACT
Long-term cell cultures (GI-LA-N and GI-ME-N) were established from the metastases of two disseminated neuroblastomas (NB). The first was obtained from a lymph node biopsy of a stage III NB after 7 months of chemotherapy, and the second from a bone marrow specimen of a stage IV NB after 6 months of chemotherapy. Cytogenetic investigation revealed several structural and numerical alterations in both cell cultures, but the only common chromosomal aberration was partial monosomy of 1p. Moreover, at the 5th in vitro passage, GI-LA-N displayed a high number of double minutes, not seen in GI-ME-N even after 33 subcultures. Molecular analysis revealed N-myc oncogene amplification in GI-LA-N cells, whereas, only one copy was found in GI-ME-N. No structural N-myc rearrangement was detected in either cell culture.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células Tumorais Cultivadas
/
Neuroblastoma
Limite:
Child, preschool
/
Humans
Idioma:
En
Revista:
Cancer Genet Cytogenet
Ano de publicação:
1988
Tipo de documento:
Article
País de afiliação:
Itália