MSI-WES: a simple approach for microsatellite instability testing using whole exome sequencing.
Future Oncol
; 17(27): 3595-3606, 2021 Sep.
Article
em En
| MEDLINE
| ID: mdl-34291669
ABSTRACT
Aim:
To demonstrate that MSI-WES is an accurate testing method for microsatellite instability (MSI). Materials &methods:
Microsatellite-based indels were counted in the variant call-formatted whole exome sequencing (WES) data of 441 gastric cancer cases using Unix-based algorithms, and the counts expressed as a fraction of the genome sequenced to obtain next-generation sequencing-based MSI indices.Results:
The next-generation sequencing-based MSI indices showed a near-perfect concordance with PCR-based MSI status, and moderate to good correlations with the molecular targets of MSI index, MLH1 expression and MLH1 methylation status, at a level comparable to the strengths of correlation between PCR-based MSI status and molecular targets of MSI index/MLH1 expression and methylation.Conclusion:
MSI-WES is a valid, adequate and sensitive approach for testing MSI in cancer.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Gástricas
/
Instabilidade de Microssatélites
/
Sequenciamento do Exoma
Limite:
Humans
Idioma:
En
Revista:
Future Oncol
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Reino Unido