Digitalis hemoperfusion: increase of column binding capacity by pretreatment with ouabain.
Biomater Artif Cells Artif Organs
; 15(3): 539-47, 1987.
Article
em En
| MEDLINE
| ID: mdl-3440130
ABSTRACT
Extracorporeal hemoperfusion through polyacrolein microsphere beads (APAMB) attached to antidigoxin antibodies is an effective treatment of digitalis intoxication. In order to increase the binding capacity of the APAMB columns the active sites of the antidigoxin antibodies were protected by ouabain during the binding to the microbeads. This brought about an 11-18 percent increase in digoxin binding capacity of the columns. It was also found that binding capacity does not increase with the rise of antibody concentration beyond a certain limit. Protection of antibody binding sites and determination of optimal concentration are, therefore necessary steps during preparation of antibody based hemoperfusion columns.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ouabaína
/
Hemoperfusão
/
Glicosídeos Digitálicos
/
Digoxina
Limite:
Humans
Idioma:
En
Revista:
Biomater Artif Cells Artif Organs
Assunto da revista:
BIOTECNOLOGIA
/
ENGENHARIA BIOMEDICA
/
TRANSPLANTE
Ano de publicação:
1987
Tipo de documento:
Article
País de afiliação:
Israel