Optimization of antigen-specific CD8+ T cell activation conditions for infectious diseases including COVID-19.
STAR Protoc
; 2(3): 100789, 2021 09 17.
Article
em En
| MEDLINE
| ID: mdl-34414379
ABSTRACT
Here, we describe the use of the artificial antigen-presenting cell (aAPC) system for the verification of T-cell epitopes. We purify and activate CD8+ T cells from blood samples from HLA-A2 that are negative for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). CD8+ T cells are combined with peptide-loaded T2-A2 cells, which are then stained with a SARS-CoV-2-specific MHC-1 tetramer to identify specific HLA-A2-restricted T-cell epitopes. The use of aAPC and healthy donors means that only BSL2 lab conditions are needed. For details of the use and implementation of this protocol, please refer to Deng et al. (2021).
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Antígeno HLA-A2
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Membro 7 da Superfamília de Receptores de Fatores de Necrose Tumoral
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Linfócitos T CD8-Positivos
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Epitopos de Linfócito T
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Glicoproteína da Espícula de Coronavírus
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SARS-CoV-2
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COVID-19
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
STAR Protoc
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
China