An engineered transcriptional reporter of protein localization identifies regulators of mitochondrial and ER membrane protein trafficking in high-throughput CRISPRi screens.

Coukos, Robert; Yao, David; Sanchez, Mateo I; Strand, Eric T; Olive, Meagan E; Udeshi, Namrata D; Weissman, Jonathan S; Carr, Steven A; Bassik, Michael C; Ting, Alice Y

Coukos, Robert; Yao, David; Sanchez, Mateo I; Strand, Eric T; Olive, Meagan E; Udeshi, Namrata D; Weissman, Jonathan S; Carr, Steven A; Bassik, Michael C; Ting, Alice Y.

Afiliação

Coukos R; Department of Genetics, Stanford University, Stanford, United States.
Yao D; Department of Genetics, Stanford University, Stanford, United States.
Sanchez MI; Department of Genetics, Stanford University, Stanford, United States.
Strand ET; Chan Zuckerberg Biohub, Stanford, United States.
Olive ME; Department of Genetics, Stanford University, Stanford, United States.
Udeshi ND; Broad Institute of MIT and Harvard, Cambridge, United States.
Weissman JS; Broad Institute of MIT and Harvard, Cambridge, United States.
Carr SA; Whitehead Institute, Cambridge, United States.
Bassik MC; Department of Biology, Massachusetts Institute of Technology, Cambridge, United States.
Ting AY; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, United States.

Elife ; 102021 08 20.

Article em En | MEDLINE | ID: mdl-34414886

Assuntos

Retículo Endoplasmático/metabolismo; Proteínas de Membrana/metabolismo; Mitocôndrias/metabolismo; Enzimas Ativadoras de Ubiquitina/metabolismo; Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas; Citometria de Fluxo; Células HEK293; Células HeLa; Humanos; Células K562; Proteínas de Membrana/genética; Transporte Proteico; Transdução de Sinais/genética; Enzimas Ativadoras de Ubiquitina/genética

Palavras-chave

CRISPR; biochemistry; cell biology; chemical biology; high-throughput screens; human; protein engineering; tail-anchored proteins

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Enzimas Ativadoras de Ubiquitina / Retículo Endoplasmático / Proteínas de Membrana / Mitocôndrias Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Elife Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos

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