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Apomorphine Reduces A53T α-Synuclein-Induced Microglial Reactivity Through Activation of NRF2 Signalling Pathway.
Heurtaux, Tony; Kirchmeyer, Melanie; Koncina, Eric; Felten, Paul; Richart, Lorraine; Uriarte Huarte, Oihane; Schohn, Herve; Mittelbronn, Michel.
Afiliação
  • Heurtaux T; Faculty of Science, Technology and Medicine, University of Luxembourg, L-4365, Esch-sur-Alzette, Luxembourg. tony.heurtaux@uni.lu.
  • Kirchmeyer M; Department of Life Sciences and Medicine (DLSM), University of Luxembourg, 7, Avenue des Hauts Fourneaux, L-4362, Esch-sur-Alzette, Luxembourg. tony.heurtaux@uni.lu.
  • Koncina E; Luxembourg Center of Neuropathology (LCNP), L-3555, Dudelange, Luxembourg. tony.heurtaux@uni.lu.
  • Felten P; Faculty of Science, Technology and Medicine, University of Luxembourg, L-4365, Esch-sur-Alzette, Luxembourg.
  • Richart L; Department of Life Sciences and Medicine (DLSM), University of Luxembourg, 7, Avenue des Hauts Fourneaux, L-4362, Esch-sur-Alzette, Luxembourg.
  • Uriarte Huarte O; Faculty of Science, Technology and Medicine, University of Luxembourg, L-4365, Esch-sur-Alzette, Luxembourg.
  • Schohn H; Department of Life Sciences and Medicine (DLSM), University of Luxembourg, 7, Avenue des Hauts Fourneaux, L-4362, Esch-sur-Alzette, Luxembourg.
  • Mittelbronn M; Faculty of Science, Technology and Medicine, University of Luxembourg, L-4365, Esch-sur-Alzette, Luxembourg.
Cell Mol Neurobiol ; 42(8): 2673-2695, 2022 Nov.
Article em En | MEDLINE | ID: mdl-34415465
ABSTRACT
The chiral molecule, apomorphine, is currently used for the treatment of Parkinson's disease (PD). As a potent dopamine receptor agonist, this lipophilic compound is especially effective for treating motor fluctuations in advanced PD patients. In addition to its receptor-mediated actions, apomorphine has also antioxidant and free radical scavenger activities. Neuroinflammation, oxidative stress, and microglia reactivity have emerged as central players in PD. Thus, modulating microglia activation in PD may be a valid therapeutic strategy. We previously reported that murine microglia are strongly activated upon exposure to A53T mutant α-synuclein. The present study was designed to investigate whether apomorphine enantiomers could modulate this A53T-induced microglial activation. Taken together, the results provided evidence that apomorphine enantiomers decrease A53T-induced microgliosis, through the activation of the NRF2 signalling pathway, leading to a lower pro-inflammatory state and restoring the phagocytic activity. Suppressing NRF2 recruitment (trigonelline exposure) or silencing specifically Nfe2l2 gene (siRNA treatment) abolished or strongly decreased the anti-inflammatory activity of apomorphine. In conclusion, apomorphine, which is already used in PD patients to mimic dopamine activity, may also be suitable to decrease α-synuclein-induced microglial reactivity.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doença de Parkinson / Alfa-Sinucleína Limite: Animals / Humans Idioma: En Revista: Cell Mol Neurobiol Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Luxemburgo

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doença de Parkinson / Alfa-Sinucleína Limite: Animals / Humans Idioma: En Revista: Cell Mol Neurobiol Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Luxemburgo