Easy detection of siderophore production in diluted growth media using an improved CAS reagent.
J Microbiol Methods
; 189: 106310, 2021 10.
Article
em En
| MEDLINE
| ID: mdl-34428498
ABSTRACT
Siderophores are low molecular weight organic compounds produced by various microorganisms, especially pathogenic bacteria including rhizobacteria, and have a high affinity for iron. Although most microorganisms are thought to secrete siderophores under iron-depleted conditions, it is unclear how many microorganisms produce siderophores in the natural environment. Also, the chrome azurol sulfonate (CAS) assay, which is widely used for the detection of siderophores, needs to be improved for wider applicability. We developed a simple, high-throughput CAS assay in a 96-well microplate with a concentrated CAS reagent and commonly used diluted growth media in the absence of artificial iron depletion. The improved microplate CAS shuttle assay revealed that it could easily detect siderophores released from Pseudomonas (P.) fluorescence, P. putida, Burlkholderia stabilis, and Ottowia oryzae, as models of siderophore-producing bacteria. This CAS shuttle assay employed along with diluted growth media is a promising tool to screen new siderophore-producing bacteria.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bactérias
/
Sideróforos
/
Meios de Cultura
/
Ensaios de Triagem em Larga Escala
/
Hidroxibenzoatos
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
J Microbiol Methods
Ano de publicação:
2021
Tipo de documento:
Article