Efficient and Rapid Analysis of Polysomes and Ribosomal Subunits in Cells and Tissues Using Ribo Mega-SEC.

Yoshikawa, Harunori; Sundaramoorthy, Ramasubramanian; Mariyappa, Daniel; Jiang, Hao; Lamond, Angus I

Yoshikawa, Harunori; Sundaramoorthy, Ramasubramanian; Mariyappa, Daniel; Jiang, Hao; Lamond, Angus I.

Afiliação

Yoshikawa H; Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee, United Kingdom.
Sundaramoorthy R; Division of Cell Signalling, Fujii Memorial Institute of Medical Sciences, Institute of Advanced Medical Sciences, Tokushima University, Tokushima, Japan.
Mariyappa D; Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee, United Kingdom.
Jiang H; Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee, United Kingdom.
Lamond AI; Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee, United Kingdom.

Bio Protoc ; 11(15): e4106, 2021 Aug 05.

Article em En | MEDLINE | ID: mdl-34458400

Palavras-chave

Polysome; Polysome profile; Protein synthesis; Ribosome; Size-exclusion chromatography; Sucrose density gradient; mRNA translation

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Bio Protoc Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Reino Unido

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