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CRISPR/Cas9 Ablation of Integrated HIV-1 Accumulates Proviral DNA Circles with Reformed Long Terminal Repeats.
Lai, Michele; Maori, Eyal; Quaranta, Paola; Matteoli, Giulia; Maggi, Fabrizio; Sgarbanti, Marco; Crucitta, Stefania; Pacini, Simone; Turriziani, Ombretta; Antonelli, Guido; Heeney, Jonathan L; Freer, Giulia; Pistello, Mauro.
Afiliação
  • Lai M; Retrovirus Center, Virology Section, Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisagrid.5395.a, Pisa, Italy.
  • Maori E; Laboratory of Viral Zoonotics, Department of Veterinary Medicine, University of Cambridgegrid.5335.0, Cambridge, United Kingdom.
  • Quaranta P; Laboratory of Viral Zoonotics, Department of Veterinary Medicine, University of Cambridgegrid.5335.0, Cambridge, United Kingdom.
  • Matteoli G; Retrovirus Center, Virology Section, Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisagrid.5395.a, Pisa, Italy.
  • Maggi F; Retrovirus Center, Virology Section, Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisagrid.5395.a, Pisa, Italy.
  • Sgarbanti M; Laboratory of Viral Zoonotics, Department of Veterinary Medicine, University of Cambridgegrid.5335.0, Cambridge, United Kingdom.
  • Crucitta S; Department of Medicine and Surgery, University of Insubria, Varese, Italy.
  • Pacini S; Virology Unit, Pisa University Hospital, Pisa, Italy.
  • Turriziani O; National Institute of Health, Rome, Italy.
  • Antonelli G; Pharmacology Unit, Department of Clinical and Experimental Medicine, University of Pisagrid.5395.a, Pisa, Italy.
  • Heeney JL; Hematology Unit, Department of Clinical and Experimental Medicine, University of Pisagrid.5395.a, Pisa, Italy.
  • Freer G; Laboratory of Virology, Department of Molecular Medicine, Sapienza University of Romegrid.7841.a, Rome, Italy.
  • Pistello M; Pasteur Institute-Cenci Bolognetti Foundation, Department of Molecular Medicine, Sapienza University of Romegrid.7841.a, Rome, Italy.
J Virol ; 95(23): e0135821, 2021 11 09.
Article em En | MEDLINE | ID: mdl-34549986
ABSTRACT
Gene editing may be used to excise the human immunodeficiency virus type 1 (HIV-1) provirus from the host cell genome, possibly eradicating the infection. Here, using cells acutely or latently infected by HIV-1 and treated with long terminal repeat (LTR)-targeting CRISPR/Cas9, we show that the excised HIV-1 provirus persists for a few weeks and may rearrange in circular molecules. Although circular proviral DNA is naturally formed during HIV-1 replication, we observed that gene editing might increase proviral DNA circles with restored LTRs. These extrachromosomal elements were recovered and probed for residual activity through their transfection in uninfected cells. We discovered that they can be transcriptionally active in the presence of Tat and Rev. Although confirming that gene editing is a powerful tool to eradicate HIV-1 infection, this work highlights that, to achieve this goal, the LTRs must be cleaved in several pieces to avoid residual activity and minimize the risk of reintegration in the context of genomic instability, possibly caused by the off-target activity of Cas9. IMPORTANCE The excision of HIV-1 provirus from the host cell genome has proven feasible in vitro and, to some extent, in vivo. Among the different approaches, CRISPR/Cas9 is the most promising tool for gene editing. The present study underlines the remarkable effectiveness of CRISPR/Cas9 in removing the HIV-1 provirus from infected cells and investigates the fate of the excised HIV-1 genome. This study demonstrates that the free provirus may persist in the cell after editing and in appropriate circumstances may reactivate. As an episome, it might be transcriptionally active, especially in the presence of Tat and Rev. The persistence of the HIV-1 episome was strongly decreased by gene editing with multiple targets. Although gene editing has the potential to eradicate HIV-1 infection, this work highlights a potential issue that warrants further investigation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA Circular / HIV-1 / Provírus / Sequências Repetidas Terminais / Sistemas CRISPR-Cas Limite: Humans Idioma: En Revista: J Virol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Itália

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA Circular / HIV-1 / Provírus / Sequências Repetidas Terminais / Sistemas CRISPR-Cas Limite: Humans Idioma: En Revista: J Virol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Itália