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Nutrient-specific proteomic analysis of the mucin degrading bacterium Akkermansia muciniphila.
Lee, Ji-Young; Jin, Hyeon-Su; Kim, Kyoung Su; Baek, Je-Hyun; Kim, Bong-Soo; Lee, Dong-Woo.
Afiliação
  • Lee JY; Department of Biotechnology, Yonsei University, Seoul, South Korea.
  • Jin HS; Department of Biotechnology, Yonsei University, Seoul, South Korea.
  • Kim KS; Department of Biotechnology, Yonsei University, Seoul, South Korea.
  • Baek JH; R&D Center for Clinical Mass Spectrometry, Seegene Medical Foundation, Seoul, South Korea.
  • Kim BS; Department of Life Science, Multidisciplinary Genome Institute, Hallym University, Chuncheon, South Korea.
  • Lee DW; Department of Biotechnology, Yonsei University, Seoul, South Korea.
Proteomics ; 22(3): e2100125, 2022 02.
Article em En | MEDLINE | ID: mdl-34596327
ABSTRACT
Akkermansia muciniphila is a prominent mucin-degrading bacterium that acts as a keystone species in regulating the human gut microbiota. Despite recently increasing research into this bacterium and its relevance to human health, a high-resolution database of its functional proteins remains scarce. Here, we provide a proteomic overview of A. muciniphila grown in different nutrient conditions ranging from defined to complex. Of 2318 protein-coding genes in the genome, we identified 841 (40%) that were expressed at the protein level. Overall, proteins involved in energy production and carbohydrate metabolism indicate that A. muciniphila relies mainly on the Embden-Meyerhof-Parnas pathway, and produces short-chain fatty acids through anaerobic fermentation in a nutrient-specific manner. Moreover, this bacterium possesses a broad repertoire of glycosyl hydrolases, together with putative peptidases and sulfatases, to cleave O-glycosylated mucin. Of them, putative mucin-degrading enzymes (Amuc_1220, Amuc_1120, Amuc_0052, Amuc_0480, and Amuc_0060) are highly abundant in the mucin-supplemented media. Furthermore, A. muciniphila uses mucin-derived monosaccharides as sources of energy and cell wall biogenesis. Our dataset provides nutrient-dependent global proteomes of A. muciniphila ATCC BAA-835 to offer insights into its metabolic functions that shape the composition of the human gut microbiota via mucin degradation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteômica / Mucinas Limite: Humans Idioma: En Revista: Proteomics Assunto da revista: BIOQUIMICA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Coréia do Sul

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteômica / Mucinas Limite: Humans Idioma: En Revista: Proteomics Assunto da revista: BIOQUIMICA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Coréia do Sul