Effects of cell concentration, time of fresh storage, and cryopreservation on peripheral blood stem cells: PBSC fresh storage and cryopreservation.
Transfus Apher Sci
; 61(1): 103298, 2022 Feb.
Article
em En
| MEDLINE
| ID: mdl-34696981
ABSTRACT
INTRODUCTION:
Peripheral blood stem cells are widely used in autologous or allogeneic transplantation. The quality of the product directly impacts clinical outcomes, and the cell quality and/or functionality may be influenced by the storage conditions as time, temperature, total nucleated cells (TNC) concentration and cryopreservation requirement.OBJECTIVE:
To verify the effects of time, cell concentration, and cryopreservation/thawing in the viability and functionality of stem cells for transplantation.METHODS:
We evaluated TNC, CD45+ viable cells, CD34+ viable cells, and cell viability and functionality of 11 samples. Measurements were performed immediately and 24 h, 48 h, 72 h, and 96 h after sample collection at high and low TNC concentrations. The same parameters were also evaluated after cryopreservation and thawing of the samples.RESULT:
Duration of storage and TNC concentration exhibited a negative effect on cell quality (CD45+ viable cells, CD34+ viable cells and functionality). Moreover, the association of these parameters increased the negative effect on graft quality. Cryopreservation and thawing also negatively affected the collected sample regarding viable CD34+ cells (recovery 66.2 %), viable CD45+ cells (recovery 56.8 %), and 7-AAD viability. No significant losses in viable CD45+/CD34+ cells and functionality were observed in the first 24 h in both TNC conditions.CONCLUSION:
These results emphasize the importance to consider carefully the storage conditions until transplantation, measuring TNC/µL until 24 h after collection (diluting the product when TNC > 300 × 103/µL) and infusing fresh graft as soon as possible.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Criopreservação
/
Transplante de Células-Tronco de Sangue Periférico
/
Células-Tronco de Sangue Periférico
Limite:
Adult
/
Aged
/
Humans
/
Middle aged
Idioma:
En
Revista:
Transfus Apher Sci
Assunto da revista:
HEMATOLOGIA
Ano de publicação:
2022
Tipo de documento:
Article