Cholesterol metabolism is decreased in patients with diminished ovarian reserve.
Reprod Biomed Online
; 44(1): 185-192, 2022 01.
Article
em En
| MEDLINE
| ID: mdl-34801402
ABSTRACT
RESEARCH QUESTION Does cholesterol metabolism differ in patients with diminished ovarian reserve (DOR) compared to patients with normal ovarian reserve (NOR)? DESIGN:
The current research included 72 women with NOR and 86 women with DOR. Data on the cholesterol metabolism in granulosa cells of these women were analysed.RESULTS:
On the day of human chorionic gonadotrophin injection, serum oestradiol and progesterone in the DOR group were significantly lower than in the control group (P < 0.001). There were no significant differences in serum concentrations of total cholesterol, triglyceride, high-density lipoprotein and low-density lipoprotein between the NOR and DOR groups. The cholesterol-regulated gene SCAP in granulosa cells from women with DOR was down-regulated (Pâ¯=â¯0.024). Cholesterol synthesis and transport genes (e.g. IDI1, FDFT1, CYP51A1, SRB1 and STARD1) were also significantly decreased (Pâ¯=â¯0.026, Pâ¯=â¯0.044, Pâ¯=â¯0.049, Pâ¯=â¯0.004 and P < 0.001, respectively). In granulosa cells of patients with DOR, cholesterol-related substances such as coprostanone, 11A-acetoxyprogesterone and 17α-hydroxyprogesterone were significantly reduced (Pâ¯=â¯0.0008, Pâ¯=â¯0.0269, Pâ¯=â¯0.0337, respectively). CYP19A1, a key steroidogenesis gene, was significantly reduced (Pâ¯=â¯0.009). 17α-hydroxyprogesterone and oestradiol decreased (Pâ¯=â¯0.004 and Pâ¯=â¯0.039, respectively).CONCLUSION:
Decreased cholesterol metabolism affecting steroid hormone synthesis in granulosa cells might be a possible mechanism for DOR.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doenças Ovarianas
/
Reserva Ovariana
/
Infertilidade Feminina
Limite:
Female
/
Humans
/
Male
Idioma:
En
Revista:
Reprod Biomed Online
Assunto da revista:
MEDICINA REPRODUTIVA
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
China