Enhancing cofactor regeneration of cyanobacteria for the light-powered synthesis of chiral alcohols.
Bioorg Chem
; 118: 105477, 2022 01.
Article
em En
| MEDLINE
| ID: mdl-34814084
ABSTRACT
Cyanobacteria Synechocystis sp. PCC 6803 was exploited as green cell factory for light-powered asymmetric synthesis of aromatic chiral alcohols. The effect of temperature, light, substrate and cell concentration on substrate conversions were investigated. Under the optimal condition, a series of chiral alcohols were synthesized with conversions up to 95% and enantiomer excess (ee)â¯>â¯99%. We found that the addition of Na2S2O3 and Angeli's Salt increased the NADPH content by 20% and 25%, respectively. As a result, the time to reach 95% substrate conversion was shortened by 12â¯h, which demonstrated that the NADPH regeneration and hence the reaction rates can be regulated in cyanobacteria. This blue-green algae based biocatalysis showed its potential for chiral compounds production in future.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Synechocystis
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Álcoois
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Luz
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NADP
Idioma:
En
Revista:
Bioorg Chem
Ano de publicação:
2022
Tipo de documento:
Article