Glycated Tryptophan PHP-THßC Incorporated into Various Chicken Embryonic Cells Constitutes Cellular Proteins.
J Poult Sci
; 58(4): 258-262, 2021 Oct 25.
Article
em En
| MEDLINE
| ID: mdl-34899021
ABSTRACT
Glycation is a non-enzymatic reaction, and amino acids are glycated by glucose in vivo. Tryptophan is glycated with glucose to form two types of glycated compounds, tryptophan-Amadori product and (1R, 3S)-1-(D-gluco-1, 2, 3, 4, 5-pentahydroxypentyl)-1, 2, 3, 4-tetrahydro-ß-carboline-3-carboxylic acid (PHP-THßC). Although PHP-THßC can be incorporated into various chicken embryonic cells, the mechanism of its incorporation into intracellular fluids has not been clarified. In this study, we examined whether PHP-THßC once incorporated into various chicken embryonic cells can combine with proteins. Embryonic cells from the breast muscle, liver, spleen, kidney, proventriculus, gizzard, and skin were prepared and 3H-PHP-THßC was added to the culture medium at final concentrations of 0, 200, 400, 600, and 800 µM to examine the incorporation of PHP-THßC. After 18 h of incubation, radioactivity was measured in the whole-cell and protein fractions of the chicken embryonic cells. As PHP-THßC concentration increased from 0 to 600 µM, its accumulation in the whole-cell fractions of all types of chicken embryonic cells linearly increased and reached the maximum level. The saturated PHP-THßC accumulation in the whole-cell fractions suggests that PHP-THßC could be incorporated into intracellular fluids across cellular membranes by some transporter proteins. As PHP-THßC concentration increased from 0 to 800 µM, its accumulation in the protein fractions of all types of chicken embryonic cells increased in a linear manner and reached a maximum level in the 800 µM PHPTHßC treatment group. This is the first study to indicate that a part of PHP-THßC incorporated into the whole-cell fraction was detected in the protein fraction of various chicken embryonic cells.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
J Poult Sci
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Japão