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Exploring lipophilic compounds that induce BDNF secretion in astrocytes beyond the BBB using a new multi-cultured human in vitro BBB model.
Fujisawa, Miwako; Takeshita, Yukio; Fujikawa, Susumu; Matsuo, Kinya; Okamoto, Masashi; Tamada, Masaya; Shimizu, Fumitaka; Sano, Yasuteru; Koga, Michiaki; Kanda, Takashi.
Afiliação
  • Fujisawa M; Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan. Electronic address: fuji0418@yamaguchi-u.ac.jp.
  • Takeshita Y; Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan. Electronic address: takeshy@yamaguchi-u.ac.jp.
  • Fujikawa S; Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan. Electronic address: smfujika@yamaguchi-u.ac.jp.
  • Matsuo K; Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan. Electronic address: matsuok@yamaguchi-u.ac.jp.
  • Okamoto M; Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan. Electronic address: i022eb@yamaguchi-u.ac.jp.
  • Tamada M; Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan. Electronic address: i055eb@yamaguchi-u.ac.jp.
  • Shimizu F; Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan. Electronic address: fshimizu@yamaguchi-u.ac.jp.
  • Sano Y; Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan. Electronic address: yasuteru@yamaguchi-u.ac.jp.
  • Koga M; Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan. Electronic address: kogamrk@yamaguchi-u.ac.jp.
  • Kanda T; Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan. Electronic address: tkanda@yamaguchi-u.ac.jp.
J Neuroimmunol ; 362: 577783, 2022 01 15.
Article em En | MEDLINE | ID: mdl-34902709
Brain-derived neurotrophic factor (BDNF) cannot cross the blood-brain barrier (BBB) when administered peripherally, which hinders its therapeutic potential. We utilized an in vitro BBB model-a tri-culture of a human endothelial cell line, a pericyte cell line, and an astrocyte cell line-to study the effect of twenty candidate lipophilic compounds on stimulating BDNF secretion in pericytes and astrocytes. The prostaglandin E2 receptor 4 agonist and sphingosine-1-phosphate receptor 5 agonist facilitated secretion of BDNF in the astrocyte, but did not decrease the transendothelial electrical resistance. These compounds may be promising agents for neurodegenerative and neuroinflammatory diseases.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Barreira Hematoencefálica / Astrócitos / Técnicas de Cocultura / Fator Neurotrófico Derivado do Encéfalo Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: J Neuroimmunol Ano de publicação: 2022 Tipo de documento: Article
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Barreira Hematoencefálica / Astrócitos / Técnicas de Cocultura / Fator Neurotrófico Derivado do Encéfalo Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: J Neuroimmunol Ano de publicação: 2022 Tipo de documento: Article