Assessment of Zinc Nanoparticle Effect and Expression of Zinc Uptake Gene in Drug Resistance Acinetobacter baumannii Strain FMHLN5.

ABSTRACT

BACKGROUND: Drug resistance bacteria pose an increasing threat to public health. Antimicrobial agents often lack efficacy toward recently developed drug resistance bacteria. Nanoparticles are one of the most effective treatment agents. The aim of this study was to evaluate the expression of zinc uptake regulator gene with zinc nanoparticle stress in drug resistant Acinetobacter baumannii strain FMHLN5. METHODS: The antimicrobial susceptibility technique was evaluated through disk diffusion methods. ZnO nanoparticles (ZnO-NPs) were synthesized using an acetate precursor-based sol-gel route. In vitro, the FMHLN5 strain susceptibility to ZnO-NPs has been tested by the agar wells diffusion method, using varying sizes (20 to 520 nm) of ZnO NPs. The purities and sizes of Nano-ZnO was determined by X-ray diffraction (XRD) and scanning electron microscope (SEM). The expression of the zinc-uptake gene was investigated through the qRT-PCR technique. RESULTS: The results revealed that the ZnO NPs against clinical FMHLN5 strain were useful at different sizes. The expression of the zinc-uptake gene was observed. CONCLUSIONS: The effect of ZnO NPs was strong, as reflected by inhibition zones at different sizes. Thus, ZnO NPs potentially induced bactericidal effect for fighting FMHLN5 strain.