Long-term cultivation of canine keratinocytes.
J Invest Dermatol
; 88(2): 202-6, 1987 Feb.
Article
em En
| MEDLINE
| ID: mdl-3492569
ABSTRACT
The growth characteristics and morphology of canine keratinocytes grown in vitro were studied. Keratinocytes from canine oral mucosa, ear skin, and ventral abdominal skin were grown in culture either as explants or as dispase/trypsin-derived suspensions in the absence of a feeder cell layer. Cholera toxin and epidermal growth factor were essential to the successful long-term growth and propagation of the cells during multiple passages. Keratinocytes from all tissue sources, either as primary cultures or subcultivated for up to 10 passages, had growth characteristic and morphology similar to that reported in other species. The use of cultured canine keratinocytes should provide a suitable model for comparative in vitro studies of the pathogenesis of dermatologic diseases.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas de Cultura
/
Células Epidérmicas
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Invest Dermatol
Ano de publicação:
1987
Tipo de documento:
Article