Developmental principles informing human pluripotent stem cell differentiation to cartilage and bone.
Semin Cell Dev Biol
; 127: 17-36, 2022 07.
Article
em En
| MEDLINE
| ID: mdl-34949507
ABSTRACT
Human pluripotent stem cells can differentiate into any cell type given appropriate signals and hence have been used to research early human development of many tissues and diseases. Here, we review the major biological factors that regulate cartilage and bone development through the three main routes of neural crest, lateral plate mesoderm and paraxial mesoderm. We examine how these routes have been used in differentiation protocols that replicate skeletal development using human pluripotent stem cells and how these methods have been refined and improved over time. Finally, we discuss how pluripotent stem cells can be employed to understand human skeletal genetic diseases with a developmental origin and phenotype, and how developmental protocols have been applied to gain a better understanding of these conditions.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco Pluripotentes
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Semin Cell Dev Biol
Assunto da revista:
EMBRIOLOGIA
Ano de publicação:
2022
Tipo de documento:
Article