Low temperature combined with high inoculum density improves alpha-linolenic acid production and biochemical characteristics of Chlamydomonas reinhardtii.
Bioresour Technol
; 348: 126746, 2022 Mar.
Article
em En
| MEDLINE
| ID: mdl-35065224
ABSTRACT
Chlamydomonas reinhardtii grows fast and is rich in polyunsaturated fatty acids. To explore whether the alpha-linolenic acid (ALA) content can be further enhanced, the cultures were incubated under different culture temperatures, light intensities and inoculum densities. Results showed that temperature exhibited more great impact on ALA synthesis of C. reinhardtii than light intensity and inoculum size. The changes of light intensity and inoculum size displayed non-significant effects on ALA content. The optimal ALA proportion in cells was obtained under the condition of 10 °C, 50 µE/m2/s and 5% inoculum density, which reached â¼ 39%.The augmented initial inoculum density could markedly improve the biomass of C. reinhardtii under 10 °C. The maximum ALA productivity (16.42 mg/L/d) was gained under 10 °C coupled with 25% inoculum size, where higher intracellular sugar and protein yield were observed. These results suggest C. reinhardtii would be an alternative feedstock for the industrial production of ALA.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Chlamydomonas reinhardtii
Idioma:
En
Revista:
Bioresour Technol
Assunto da revista:
ENGENHARIA BIOMEDICA
Ano de publicação:
2022
Tipo de documento:
Article