Your browser doesn't support javascript.
loading
Engineered Chimeras Unveil Swappable Modular Features of Fatty Acid and Polyketide Synthase Acyl Carrier Proteins.
Cho, Yae In; Armstrong, Claire L; Sulpizio, Ariana; Acheampong, Kofi K; Banks, Kameron N; Bardhan, Oishi; Churchill, Sydney J; Connolly-Sporing, Annie E; Crawford, Callie E W; Cruz Parrilla, Peter L; Curtis, Sarah M; De La Ossa, Lauren M; Epstein, Samuel C; Farrehi, Clara J; Hamrick, Grayson S; Hillegas, William J; Kang, Austin; Laxton, Olivia C; Ling, Joie; Matsumura, Sara M; Merino, Victoria M; Mukhtar, Shahla H; Shah, Neel J; Londergan, Casey H; Daly, Clyde A; Kokona, Bashkim; Charkoudian, Louise K.
Afiliação
  • Cho YI; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States.
  • Armstrong CL; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States.
  • Sulpizio A; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States.
  • Acheampong KK; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States.
  • Banks KN; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States.
  • Bardhan O; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States.
  • Churchill SJ; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States.
  • Connolly-Sporing AE; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States.
  • Crawford CEW; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States.
  • Cruz Parrilla PL; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States.
  • Curtis SM; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States.
  • De La Ossa LM; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States.
  • Epstein SC; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States.
  • Farrehi CJ; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States.
  • Hamrick GS; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States.
  • Hillegas WJ; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States.
  • Kang A; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States.
  • Laxton OC; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States.
  • Ling J; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States.
  • Matsumura SM; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States.
  • Merino VM; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States.
  • Mukhtar SH; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States.
  • Shah NJ; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States.
  • Londergan CH; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States.
  • Daly CA; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States.
  • Kokona B; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States.
  • Charkoudian LK; Spark Therapeutics, Philadelphia, Pennsylvania 19041, United States.
Biochemistry ; 61(4): 217-227, 2022 02 15.
Article em En | MEDLINE | ID: mdl-35073057
The strategic redesign of microbial biosynthetic pathways is a compelling route to access molecules of diverse structure and function in a potentially environmentally sustainable fashion. The promise of this approach hinges on an improved understanding of acyl carrier proteins (ACPs), which serve as central hubs in biosynthetic pathways. These small, flexible proteins mediate the transport of molecular building blocks and intermediates to enzymatic partners that extend and tailor the growing natural products. Past combinatorial biosynthesis efforts have failed due to incompatible ACP-enzyme pairings. Herein, we report the design of chimeric ACPs with features of the actinorhodin polyketide synthase ACP (ACT) and of the Escherichia coli fatty acid synthase (FAS) ACP (AcpP). We evaluate the ability of the chimeric ACPs to interact with the E. coli FAS ketosynthase FabF, which represents an interaction essential to building the carbon backbone of the synthase molecular output. Given that AcpP interacts with FabF but ACT does not, we sought to exchange modular features of ACT with AcpP to confer functionality with FabF. The interactions of chimeric ACPs with FabF were interrogated using sedimentation velocity experiments, surface plasmon resonance analyses, mechanism-based cross-linking assays, and molecular dynamics simulations. Results suggest that the residues guiding AcpP-FabF compatibility and ACT-FabF incompatibility may reside in the loop I, α-helix II region. These findings can inform the development of strategic secondary element swaps that expand the enzyme compatibility of ACPs across systems and therefore represent a critical step toward the strategic engineering of "un-natural" natural products.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteína de Transporte de Acila / Proteínas de Escherichia coli / Policetídeo Sintases / Ácido Graxo Sintases Idioma: En Revista: Biochemistry Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteína de Transporte de Acila / Proteínas de Escherichia coli / Policetídeo Sintases / Ácido Graxo Sintases Idioma: En Revista: Biochemistry Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Estados Unidos