Production of human spinal-cord organoids recapitulating neural-tube morphogenesis.
Nat Biomed Eng
; 6(4): 435-448, 2022 04.
Article
em En
| MEDLINE
| ID: mdl-35347276
ABSTRACT
Human spinal-cord-like tissues induced from human pluripotent stem cells are typically insufficiently mature and do not mimic the morphological features of neurulation. Here, we report a three-dimensional culture system and protocol for the production of human spinal-cord-like organoids (hSCOs) recapitulating the neurulation-like tube-forming morphogenesis of the early spinal cord. The hSCOs exhibited neurulation-like tube-forming morphogenesis, cellular differentiation into the major types of spinal-cord neurons as well as glial cells, and mature synaptic functional activities, among other features of the development of the spinal cord. We used the hSCOs to screen for antiepileptic drugs that can cause neural-tube defects. hSCOs may also facilitate the study of the development of the human spinal cord and the modelling of diseases associated with neural-tube defects.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neurulação
/
Defeitos do Tubo Neural
Limite:
Humans
Idioma:
En
Revista:
Nat Biomed Eng
Ano de publicação:
2022
Tipo de documento:
Article