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Recombineering and MAGE.
Wannier, Timothy M; Ciaccia, Peter N; Ellington, Andrew D; Filsinger, Gabriel T; Isaacs, Farren J; Javanmardi, Kamyab; Jones, Michaela A; Kunjapur, Aditya M; Nyerges, Akos; Pal, Csaba; Schubert, Max G; Church, George M.
Afiliação
  • Wannier TM; Department of Genetics, Harvard Medical School, Boston, MA, USA.
  • Ciaccia PN; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA.
  • Ellington AD; Department of Molecular, Cellular & Developmental Biology, Yale University, New Haven, CT, USA.
  • Filsinger GT; Systems Biology Institute, Yale University, West Haven, CT, USA.
  • Isaacs FJ; Department of Molecular Biosciences, College of Natural Sciences, University of Texas at Austin, Austin, TX, USA.
  • Javanmardi K; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA.
  • Jones MA; Department of Systems Biology, Harvard University, Cambridge, MA, USA.
  • Kunjapur AM; Department of Molecular, Cellular & Developmental Biology, Yale University, New Haven, CT, USA.
  • Nyerges A; Systems Biology Institute, Yale University, West Haven, CT, USA.
  • Pal C; Department of Biomedical Engineering, Yale University, New Haven, CT, USA.
  • Schubert MG; Department of Molecular Biosciences, College of Natural Sciences, University of Texas at Austin, Austin, TX, USA.
  • Church GM; Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE, USA.
Nat Rev Methods Primers ; 12021.
Article em En | MEDLINE | ID: mdl-35540496
ABSTRACT
Recombination-mediated genetic engineering, also known as recombineering, is the genomic incorporation of homologous single-stranded or double-stranded DNA into bacterial genomes. Recombineering and its derivative methods have radically improved genome engineering capabilities, perhaps none more so than multiplex automated genome engineering (MAGE). MAGE is representative of a set of highly multiplexed single-stranded DNA-mediated technologies. First described in Escherichia coli, both MAGE and recombineering are being rapidly translated into diverse prokaryotes and even into eukaryotic cells. Together, this modern set of tools offers the promise of radically improving the scope and throughput of experimental biology by providing powerful new methods to ease the genetic manipulation of model and non-model organisms. In this Primer, we describe recombineering and MAGE, their optimal use, their diverse applications and methods for pairing them with other genetic editing tools. We then look forward to the future of genetic engineering.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Nat Rev Methods Primers Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Nat Rev Methods Primers Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos