Purification-induced damage to calicivirus particles at near-atomic resolution.
J Gen Virol
; 103(5)2022 05.
Article
em En
| MEDLINE
| ID: mdl-35579608
ABSTRACT
The purification of virus particles is an essential process for the manufacture of vaccines. However, the application of different purification processes may affect the quality of the virus particles, such as structural integrity and homogeneity, which may further influence the infectivity and immunogenicity of the purified virus. In this study, we took Feline calicivirus (FCV), a common natural pathogen in cats belonging to Caliciviridae, as a research model. By using cryo-electron microscopy (cryo-EM), we incorporated the 3D classification process as a virus flexibility evaluation system. Cryo-EM images of virus particles resulting from different purification processes were compared at near-atomic resolution. The results indicated that molecular sieving purification will impact the stability of P-domains through increasing flexibility as determined by the evaluation system, which can be extended to assess the purification effect on the entire particle. This evaluation process can be further applied to all non-enveloped viruses.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírus
/
Caliciviridae
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Doenças do Gato
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Calicivirus Felino
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Infecções por Caliciviridae
Limite:
Animals
Idioma:
En
Revista:
J Gen Virol
Ano de publicação:
2022
Tipo de documento:
Article