CRISPR/Cas9 gRNA activity depends on free energy changes and on the target PAM context.

Corsi, Giulia I; Qu, Kunli; Alkan, Ferhat; Pan, Xiaoguang; Luo, Yonglun; Gorodkin, Jan

CRISPR/Cas9 gRNA activity depends on free energy changes and on the target PAM context.

Corsi, Giulia I; Qu, Kunli; Alkan, Ferhat; Pan, Xiaoguang; Luo, Yonglun; Gorodkin, Jan.

Afiliação

Corsi GI; Center for non-coding RNA in Technology and Health, Department of Veterinary and Animal Sciences, University of Copenhagen, Thorvaldsensvej 57, 1871, Frederiksberg, Denmark.
Qu K; Lars Bolund Institute of Regenerative Medicine, Qingdao-Europe Advanced Institute for Life Sciences, BGI-Qingdao, Qingdao, 266555, China.
Alkan F; Department of Biology, University of Copenhagen, Copenhagen, 2200, Denmark.
Pan X; Center for non-coding RNA in Technology and Health, Department of Veterinary and Animal Sciences, University of Copenhagen, Thorvaldsensvej 57, 1871, Frederiksberg, Denmark.
Luo Y; Division of Oncogenomics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands.
Gorodkin J; Lars Bolund Institute of Regenerative Medicine, Qingdao-Europe Advanced Institute for Life Sciences, BGI-Qingdao, Qingdao, 266555, China.

Nat Commun ; 13(1): 3006, 2022 05 30.

Article em En | MEDLINE | ID: mdl-35637227

Assuntos

Sistemas CRISPR-Cas; RNA Guia de Cinetoplastídeos; Genoma; RNA Guia de Cinetoplastídeos/genética

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: RNA Guia de Cinetoplastídeos / Sistemas CRISPR-Cas Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Dinamarca

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