ATP Hydrolysis Coordinates the Activities of Two Motors in a Dimeric Chromatin Remodeling Enzyme.
J Mol Biol
; 434(14): 167653, 2022 07 30.
Article
em En
| MEDLINE
| ID: mdl-35659534
ABSTRACT
ATP-dependent chromatin remodelers are essential enzymes that restructure eukaryotic genomes to enable all DNA-based processes. The diversity and complexity of these processes arethe complexity of the enzymes that carry them out, making remodelers a challenging class of molecular motors to study by conventional methods. Here we use a single molecule biophysical assay to overcome some of these challenges, enabling a detailed mechanistic dissection of a paradigmatic remodeler reaction, that of sliding a nucleosome towards the longer DNA linker. We focus on how two motors of a dimeric remodeler coordinate to accomplish such directional sliding. We find that ATP hydrolysis by both motors promotes coordination, suggesting a role for ATP in resolving the competition for directional commitment. Furthermore, we show an artificially constitutive dimer is no more or less coordinated, but is more processive, suggesting a cell could modulate a remodeler's oligomeric state to modulate local chromatin dynamics.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromatina
/
Montagem e Desmontagem da Cromatina
Idioma:
En
Revista:
J Mol Biol
Ano de publicação:
2022
Tipo de documento:
Article