Immunoperoxidase localization of vitamin D dependent calcium binding protein.
J Histochem Cytochem
; 26(8): 628-34, 1978 Aug.
Article
em En
| MEDLINE
| ID: mdl-357647
Calcium binding protein (CaBP) was localized by the indirect peroxidase-labeled antibody method in chick duodenum 72 hr after administering 32.5 nmol of cholecalciferol to vitamin D-deficient chicks. CaBP was observed in cytoplasm and nuclei of absorptive cells but was absent from goblet cells. Our results are consistent with the suggested functional role for CaBP in the prevention of intracellular accumulation of calcium by preventing mitochondrial accumulation of calcium, enhancing removal of calcium from absorptive cells, and/or preventing the "leaking" of calcium into cells through the lateral borders. They are not consistent with an extracellular functional role for CaBP.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteína G de Ligação ao Cálcio S100
/
Proteínas de Transporte
/
Técnicas Imunoenzimáticas
/
Duodeno
Limite:
Animals
Idioma:
En
Revista:
J Histochem Cytochem
Assunto da revista:
HISTOCITOQUIMICA
Ano de publicação:
1978
Tipo de documento:
Article