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Chemogenetic inhibition of corticotropin-releasing factor neurons in the central amygdala alters binge-like ethanol consumption in male mice.
Marshall, S Alex; Robinson, Stacey L; Ebert, Suzahn E; Companion, Michel A; Thiele, Todd E.
Afiliação
  • Marshall SA; Department of Psychology & Neuroscience.
  • Robinson SL; Department of Psychology & Neuroscience.
  • Ebert SE; Department of Psychology & Neuroscience.
  • Companion MA; Department of Psychology & Neuroscience.
  • Thiele TE; Department of Psychology & Neuroscience.
Behav Neurosci ; 136(6): 541-550, 2022 Dec.
Article em En | MEDLINE | ID: mdl-35771510
Repetitive bouts of binge drinking can lead to neuroplastic events that alter ethanol's pharmacologic effects and perpetuate excessive consumption. The corticotropin-releasing factor (CRF) system is an example of ethanol-induced neuroadaptations that drive excessive ethanol consumption. Our laboratory has previously shown that CRF antagonist, when infused into the central amygdala (CeA), reduces binge-like ethanol consumption. The present study extends this research by assessing the effects of silencing CRF-producing neurons in CeA on binge-like ethanol drinking stemming from "Drinking in the Dark" (DID) procedures. CRF-ires-Cre mice underwent surgery to infuse Gi/o-coupled Designer Receptors Exclusively Activated by Designer Drugs (DREADD) virus or a control virus into either the CeA or basolateral amygdala (BLA). Gi/o-DREADD-induced CRF-neuronal inhibition in the CeA resulted in a 33% decrease in binge-like ethanol consumption. However, no effect on ethanol consumption was seen after DREADD manipulation in the BLA. Moreover, CeA CRF-neuronal inhibition had no effect on sucrose consumption. The effects of silencing CRF neurons in the CeA on ethanol consumption are not secondary to changes in motor function or anxiety-like behaviors as assessed in the open-field test (OFT). Finally, the DREADD construct's functional ability to inhibit CRF-neuronal activity was demonstrated by reduced ethanol-induced c-Fos following DREADD activation. Together, these data suggest that the CRF neurons in the CeA play an important role in binge ethanol consumption and that inhibition of the CRF-signaling pathway remains a viable target for manipulating binge-like ethanol consumption. (PsycInfo Database Record (c) 2022 APA, all rights reserved).
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Hormônio Liberador da Corticotropina / Núcleo Central da Amígdala Limite: Animals Idioma: En Revista: Behav Neurosci Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Hormônio Liberador da Corticotropina / Núcleo Central da Amígdala Limite: Animals Idioma: En Revista: Behav Neurosci Ano de publicação: 2022 Tipo de documento: Article