ProD: A Tool for Predictive Design of Tailored Promoters in Escherichia coli.
Methods Mol Biol
; 2516: 51-59, 2022.
Article
em En
| MEDLINE
| ID: mdl-35922621
ABSTRACT
A major goal in synthetic biology is the engineering of synthetic gene circuits with a predictable, controlled and designed outcome. This creates a need for building blocks that can modulate gene expression without interference with the native cell system. A tool allowing forward engineering of promoters with predictable transcription initiation frequency is still lacking. Promoter libraries specific for σ70 to ensure the orthogonality of gene expression were built in Escherichia coli and labeled using fluorescence-activated cell sorting to obtain high-throughput DNA sequencing data to train a convolutional neural network. We were able to confirm in vivo that the model is able to predict the promoter transcription initiation frequency (TIF) of new promoter sequences. Here, we provide an online tool for promoter design (ProD) in E. coli, which can be used to tailor output sequences of desired promoter TIF or predict the TIF of a custom sequence.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Escherichia coli
/
Escherichia coli
Tipo de estudo:
Prognostic_studies
/
Risk_factors_studies
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Bélgica