[Membrane potentials of different cell types of the head of the pancreas in situ in guinea pigs]. / Membrannye potentsialy raznykh tipov kletok golovki podzheludochnoi zhelezy in situ u morskikh svinok.

ABSTRACT

Membrane potentials of different types of cells in the pancreatic head of guinea-pigs were studied in vivo, using intracellular microelectrode technique. Cellular distribution was determined at different depths 0-150 microns, 150-500 microns, 500-900 microns. It was established that the maximum number of low polarized cells (30.2%) with membrane potential (MP) up to 30 mV and a smaller number of highly polarized cells (25.8%) were situated in the external layer of the dorsal gland areas. There is a trend towards the increase in the number of highly polarized cells (35.2%) with simultaneous reduction in the number of low polarized cells (19.6%) in the middle layer. The maximum number of highly polarized cells was situated in the deep layers because of the increase in the number of cells with MP level from 50 to 60 mV and the reduction in the number of low polarized cells (12.3%). The number of cells with MP from 30 to 50 mV in external, middle, and deep layers remains practically unchanged. It is believed that with the intact blood circulation and regulation in the pancreas it is possible not only to register various types of cells, but also to examine them in various layers.