Low-cost high-resolution photoacoustic microscopy of blood oxygenation with two laser diodes.
Biomed Opt Express
; 13(7): 3893-3903, 2022 Jul 01.
Article
em En
| MEDLINE
| ID: mdl-35991932
ABSTRACT
Optical-resolution photoacoustic microscopy (OR-PAM) has been widely used for imaging blood vessel and oxygen saturation of hemoglobin (sO2), providing high-resolution functional images of living animals in vivo. However, most of them require one or multiple bulky and costly pulsed lasers, hindering their applicability in preclinical and clinical settings. In this paper, we demonstrate a reflection-mode low-cost high-resolution OR-PAM system by using two cost-effective and compact laser diodes (LDs), achieving microvasculature and sO2 imaging with a high lateral resolution of â¼6â
µm. The cost of the excitation sources has dramatically reduced by â¼20-40 times compared to that of the pulsed lasers used in state-of-the-art OR-PAM systems. A blood phantom study was performed to show a determination coefficient R 2 of 0.96 in linear regression analysis. Experimental results of in vivo mouse ear imaging show that the proposed dual-wavelength LD-based PAM system can provide high-resolution functional images at a low cost.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Tipo de estudo:
Health_economic_evaluation
Idioma:
En
Revista:
Biomed Opt Express
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
China