Studies on the interrelated stimulation of microsomal omega-oxidation and peroxisomal beta-oxidation in rat liver with a partially hydrogenated fish oil diet.
Biochim Biophys Acta
; 920(2): 114-9, 1987 Jul 31.
Article
em En
| MEDLINE
| ID: mdl-3607073
ABSTRACT
To investigate the mechanism for initiation of peroxisomal beta-oxidation by high-fat diets the time-courses of peroxisomal beta-oxidation and microsomal omega-oxidation stimulated by 20% (w/w) partially hydrogenated fish oil were studied. The relative stimulation of these two activities developed in a very similar way. We also observed an elevated level of long-chain acyl-CoA with partially hydrogenated fish oil, but not of free fatty acids. There was, however, a significant shift in the composition of free fatty acids to a higher amount of monoenes and lower amounts of 182 and 204 fatty acids. In peroxisomes purified by Nycodenz gradient centrifugation there was no lauric acid hydroxylation. This study indicates that with partially hydrogenated fish oil we obtain a parallel stimulation of reactions in two different cellular compartments. Dicarboxylic fatty acids, which are products of the omega-oxidation, had only a slight stimulatory effect on peroxisomal beta-oxidation. Therefore, the primary stimulatory agent of peroxisomal beta-oxidation and microsomal omega-oxidation is still unknown. It was speculated that this agent may activate a gene-locus responsible for both reactions.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Óleos de Peixe
/
Microssomos Hepáticos
/
Gorduras na Dieta
/
Ácidos Graxos
/
Fígado
/
Microcorpos
Limite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
1987
Tipo de documento:
Article