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Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood.
Sigal, Natalia; Maecker, Holden T.
Afiliação
  • Sigal N; Institute for Immunity, Transplantation, and Infection, Stanford University School of Medicine, Stanford, CA, USA.
  • Maecker HT; Institute for Immunity, Transplantation, and Infection, Stanford University School of Medicine, Stanford, CA, USA. maecker@stanford.edu.
Methods Mol Biol ; 2543: 113-128, 2022.
Article em En | MEDLINE | ID: mdl-36087263
Phosphoflow is a powerful tool that allows researchers to measure distinct signaling responses to various stimuli in multiple subpopulations of cells. Extension of this technique to mass cytometry (cytometry by time-of-flight or CyTOF) allows many more cell phenotypes and signaling nodes to be interrogated in parallel. The use of fresh whole blood is ideal for capturing the in vivo signaling state of all leukocytes, including granulocytes. In this chapter, we provide a detailed protocol for performing CyTOF phosphoflow in human whole blood, using cytokines and other stimuli. Barcoding and combining of multiple samples and other techniques to reduce batch effects and provide optimal comparability between samples/stimulations are also described.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Testes Imunológicos / Transdução de Sinais Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Testes Imunológicos / Transdução de Sinais Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Estados Unidos