A conserved role of bam in maintaining metabolic homeostasis via regulating intestinal microbiota in Drosophila.
PeerJ
; 10: e14145, 2022.
Article
em En
| MEDLINE
| ID: mdl-36248714
Background: Previous studies have proven that bag-of-marbles (bam) plays a pivotal role in promoting early germ cell differentiation in Drosophila ovary. However, whether it functions in regulating the metabolic state of the host remains largely unknown. Methods: We utilized GC-MS, qPCR, and some classical kits to examine various metabolic profiles and gut microbial composition in bam loss-of-function mutants and age-paired controls. We performed genetic manipulations to explore the tissue/organ-specific role of bam in regulating energy metabolism in Drosophila. The DSS-induced mouse colitis was generated to identify the role of Gm114, the mammalian homolog of bam, in modulating intestinal homeostasis. Results: We show that loss of bam leads to an increased storage of energy in Drosophila. Silence of bam in intestines results in commensal microbial dysbiosis and metabolic dysfunction of the host. Moreover, recovery of bam expression in guts almost rescues the obese phenotype in bam loss-of-function mutants. Further examinations of mammalian Gm114 imply a similar biological function in regulating the intestinal homeostasis and energy storage with its Drosophila homolog bam. Conclusion: Our studies uncover a novel biological function of bam/Gm114 in regulating the host lipid homeostasis.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Drosophila
/
Drosophila
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Microbioma Gastrointestinal
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Homeostase
Limite:
Animals
Idioma:
En
Revista:
PeerJ
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
China