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Hematopoietic Stem and Progenitor Cell Identification and Transplantation in Zebrafish.
Fraint, Ellen; Lv, Peng; Liu, Feng; Bowman, Teresa V; Tamplin, Owen J.
Afiliação
  • Fraint E; Department of Pediatrics (Pediatric Hematology/Oncology and Cellular Therapy) and Department of Developmental and Molecular Biology, Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, NY, USA.
  • Lv P; State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
  • Liu F; Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China.
  • Bowman TV; University of Chinese Academy of Science, Beijing, China.
  • Tamplin OJ; State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
Methods Mol Biol ; 2567: 233-249, 2023.
Article em En | MEDLINE | ID: mdl-36255705
The zebrafish as a model organism is well known for its versatile genetics, rapid development, and straightforward live imaging. It is an excellent model to study hematopoiesis because of its highly conserved ontogeny and gene regulatory networks. Recently developed highly specific transgenic reporter lines have allowed direct imaging and tracking of hematopoietic stem and progenitor cells (HSPCs) in live zebrafish. These reporter lines can also be used for fluorescence-activated cell sorting (FACS) of HSPCs. Similar to mammalian models, HSPCs can be transplanted to reconstitute the entire hematopoietic system of zebrafish recipients. However, the zebrafish provides unique advantages to study HSPC biology, such as transplants into embryos and high-throughput chemical screening. This chapter will outline the methods needed to identify, isolate, and transplant HSPCs in zebrafish.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peixe-Zebra / Transplante de Células-Tronco Hematopoéticas Limite: Animals Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peixe-Zebra / Transplante de Células-Tronco Hematopoéticas Limite: Animals Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos