Vibrio ostreae sp. nov., a novel gut bacterium isolated from a Yellow Sea oyster.
Int J Syst Evol Microbiol
; 72(10)2022 Oct.
Article
em En
| MEDLINE
| ID: mdl-36269578
ABSTRACT
A Gram-stain-negative, oxidase- and catalase-positive, facultative anaerobic motile bacterium, designated strain OG9-811T, was isolated from the gut of an oyster collected in the Yellow Sea, Republic of Korea. The strain grew at 10-37 °C, pH 6.0-9.0 and with 0.5-10% (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain OG9-811T affiliated with the genus Vibrio, with the highest sequence similarity of 98.2% to Vibrio coralliilyticus ATCC BAA-450T followed by Vibrio variabilis R-40492T (98.0â%), Vibrio hepatarius LMG 20362T (97.7â%) and Vibrio neptunius LMG 20536T (97.6â%); other relatives were Vibrio tritonius JCM 16456T (97.4â%), Vibrio fluvialis NBRC 103150T (97.0â%) and Vibrio furnissii CIP 102972T (97.0â%). The complete genome of strain OG9-811T comprised two chromosomes of a total 4â807â684 bp and the G+C content was 50.2 %. Results of analysis based on the whole genome sequence showed the distinctiveness of strain OG9-811T. The average nucleotide identity (ANI) values between strain OG9-811T and the closest strains V. coralliilyticus ATCC BAA-450T, V. variabilis R-40492T, V. hepatarius LMG 20362T, V. neptunius KCTC 12702T , V. tritonius JCM 16456T, V. fluvialis ATCC 33809T and V. furnissi CIP 102972T were 73.0, 72.6, 73.3, 73.0, 72.7, 78.5 and 77.8â%, respectively, while the digital DNA-DNA hybridization values between strain OG9-811T and the above closely related strains were 20.8, 21.2, 20.8, 21.7, 20.7, 23.2 and 22.4â%, respectively. The major fatty acids of strain OG9-811T were summed feature 3 (C161 ω7c and/or C161 ω6c), summed feature 8 (C181 ω6c and/or C181 ω7c) and C160. The polar lipids contained phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Strain OG9-811T contained Q-8 as a quinone. On the basis of polyphasic taxonomic characteristics, strain OG9-811T is considered to represent a novel species, for which the name Vibrio ostreae sp. nov. is proposed. The type strain is OG9-811T (=KCTC 72623T=GDMCC 1.2610T).
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ostreidae
/
Vibrio
Limite:
Animals
Idioma:
En
Revista:
Int J Syst Evol Microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2022
Tipo de documento:
Article