Phosphorus-doped deep eutectic solvent-derived carbon dots-modified silica as a mixed-mode stationary phase for reversed-phase and hydrophilic interaction chromatography.
Anal Bioanal Chem
; 415(18): 4255-4264, 2023 Jul.
Article
em En
| MEDLINE
| ID: mdl-36350343
ABSTRACT
In this work, phosphorus-doped carbon dots (P-DESCDs) were successfully prepared using choline chloride/lactic acid type deep eutectic solvent and phosphoric acid as ingredients, and (3-aminopropyl) trimethoxysilane was used as a bridge to graft P-DESCDs onto the silica surface to obtain a new mixed-mode stationary phase (Sil-P-DESCDs) for reversed-phase and hydrophilic interaction liquid chromatography. The successful preparation of the stationary phase was confirmed by laser scanning confocal microscopy, elemental analysis, and Fourier transform infrared spectrometry. Interestingly, the doping of phosphorus greatly improved the separation performance and hydrophilicity of the Sil-P-DESCDs column. The Sil-P-DESCDs column was found to have certain hydrophobicity, hydrogen bonding ability and shape selectivity by Tanaka and Engelhardt standard test mixtures, and a series of hydrophilic and hydrophobic compounds such as alkylbenzenes, polycyclic aromatic hydrocarbons, sulfonamides, aromatic amines, phenols, flavonoids, nucleoside bases, and alkaloids. In addition, the effects of mobile phase ratio, column temperature, flow rate, salt concentration, and pH on the retention of analytes on Sil-P-DESCDs columns were investigated. Finally, the Sil-P-DESCDs column was applied to the qualitative and quantitative analysis of calcein-7-glucoside in the real sample of medicinal Astragalus pellets, and it was found at a concentration of 0.02 mg/mL.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Dióxido de Silício
/
Solventes Eutéticos Profundos
Tipo de estudo:
Qualitative_research
Idioma:
En
Revista:
Anal Bioanal Chem
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
China