Identification of s9ap used as an endogenous reference gene in qualitative and real-time quantitative PCR detection of Pleurotus eryngii.
Mol Biol Rep
; 50(1): 621-629, 2023 Jan.
Article
em En
| MEDLINE
| ID: mdl-36370299
ABSTRACT
BACKGROUND:
Pleurotus eryngii is a kind of edible fungi with good quality, and it is popular among consumers. At present, some adulterated edible fungi are available in the market. The rights and interests of consumers can be ensured by establishing a practical edible fungi detection system. Among the existing methods for detecting food adulteration, endogenous reference gene amplification is convenient and reliable. However, no ideal endogenous reference gene is available for P. eryngii. METHODS ANDRESULTS:
In this study, s9ap was screened as an endogenous reference gene through sequence alignment. Qualitative and quantitative PCR analysis of this gene was carried out in one P. eryngii variety and 18 other species. The detection limit of quantitative PCR was 400 pg, and no s9ap amplification products were detected in the 18 other species.CONCLUSIONS:
This study confirmed that s9ap was an ideal endogenous reference gene for the detection of P. eryngii. This method was also suitable for processed food products.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pleurotus
Tipo de estudo:
Diagnostic_studies
/
Qualitative_research
Idioma:
En
Revista:
Mol Biol Rep
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
China