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High-throughput morphometric and transcriptomic profiling uncovers composition of naïve and sensory-deprived cortical cholinergic VIP/CHAT neurons.
Yayon, Nadav; Amsalem, Oren; Zorbaz, Tamara; Yakov, Or; Dubnov, Serafima; Winek, Katarzyna; Dudai, Amir; Adam, Gil; Schmidtner, Anna K; Tessier-Lavigne, Marc; Renier, Nicolas; Habib, Naomi; Segev, Idan; London, Michael; Soreq, Hermona.
Afiliação
  • Yayon N; The Edmond and Lily Safra Center for Brain Sciences (ELSC), The Life Sciences Institute, The Hebrew University of Jerusalem, Jerusalem, Israel.
  • Amsalem O; The Department of Biological Chemistry, The Life Sciences Institute, The Hebrew University of Jerusalem, Jerusalem, Israel.
  • Zorbaz T; The Edmond and Lily Safra Center for Brain Sciences (ELSC), The Life Sciences Institute, The Hebrew University of Jerusalem, Jerusalem, Israel.
  • Yakov O; The Department of Neurobiology, The Life Sciences Institute, The Hebrew University of Jerusalem, Jerusalem, Israel.
  • Dubnov S; The Edmond and Lily Safra Center for Brain Sciences (ELSC), The Life Sciences Institute, The Hebrew University of Jerusalem, Jerusalem, Israel.
  • Winek K; The Department of Biological Chemistry, The Life Sciences Institute, The Hebrew University of Jerusalem, Jerusalem, Israel.
  • Dudai A; Biochemistry and Organic Analytical Chemistry Unit, The Institute of Medical Research and Occupational Health, Zagreb, Croatia.
  • Adam G; The Department of Biological Chemistry, The Life Sciences Institute, The Hebrew University of Jerusalem, Jerusalem, Israel.
  • Schmidtner AK; The Edmond and Lily Safra Center for Brain Sciences (ELSC), The Life Sciences Institute, The Hebrew University of Jerusalem, Jerusalem, Israel.
  • Tessier-Lavigne M; The Department of Biological Chemistry, The Life Sciences Institute, The Hebrew University of Jerusalem, Jerusalem, Israel.
  • Renier N; The Edmond and Lily Safra Center for Brain Sciences (ELSC), The Life Sciences Institute, The Hebrew University of Jerusalem, Jerusalem, Israel.
  • Habib N; The Department of Biological Chemistry, The Life Sciences Institute, The Hebrew University of Jerusalem, Jerusalem, Israel.
  • Segev I; The Edmond and Lily Safra Center for Brain Sciences (ELSC), The Life Sciences Institute, The Hebrew University of Jerusalem, Jerusalem, Israel.
  • London M; The Department of Neurobiology, The Life Sciences Institute, The Hebrew University of Jerusalem, Jerusalem, Israel.
  • Soreq H; The Department of Biological Chemistry, The Life Sciences Institute, The Hebrew University of Jerusalem, Jerusalem, Israel.
EMBO J ; 42(1): e110565, 2023 01 04.
Article em En | MEDLINE | ID: mdl-36377476
Cortical neuronal networks control cognitive output, but their composition and modulation remain elusive. Here, we studied the morphological and transcriptional diversity of cortical cholinergic VIP/ChAT interneurons (VChIs), a sparse population with a largely unknown function. We focused on VChIs from the whole barrel cortex and developed a high-throughput automated reconstruction framework, termed PopRec, to characterize hundreds of VChIs from each mouse in an unbiased manner, while preserving 3D cortical coordinates in multiple cleared mouse brains, accumulating thousands of cells. We identified two fundamentally distinct morphological types of VChIs, bipolar and multipolar that differ in their cortical distribution and general morphological features. Following mild unilateral whisker deprivation on postnatal day seven, we found after three weeks both ipsi- and contralateral dendritic arborization differences and modified cortical depth and distribution patterns in the barrel fields alone. To seek the transcriptomic drivers, we developed NuNeX, a method for isolating nuclei from fixed tissues, to explore sorted VChIs. This highlighted differentially expressed neuronal structural transcripts, altered exitatory innervation pathways and established Elmo1 as a key regulator of morphology following deprivation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Lobo Parietal / Transcriptoma Limite: Animals Idioma: En Revista: EMBO J Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Israel

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Lobo Parietal / Transcriptoma Limite: Animals Idioma: En Revista: EMBO J Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Israel